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验证数据展示

  • WB analysis of mouse brain using 22214-1-AP

    WB analysis of mouse brain using 22214-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22214-1-AP

    IHC staining of human breast cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22214-1-AP

    IHC staining of human breast cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACP1 Polyclonal antibody

ACP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22214-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

22214-1-AP targets ACP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag17539

Product name: Recombinant human ACP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 15-102 aa of BC007422

Sequence: GNICRSPIAEAVFRKLVTDQNISENWVIDSGAVSDWNVGRSPDPRAVSCLRNHGIHTAHKARQITKEDFATFDYILCMDESNLRDLNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acid phosphatase 1, soluble
别名 acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1
计算分子量 158 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC007422
基因名称 ACP1
Gene ID (NCBI) 52
RRIDAB_2879032
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24666
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human red cell acid phosphatase (ACP1) is a polymorphic enzyme closely related to cytosolic low molecular weight acid phosphatases, a protein family broadly conserved among eukaryotes. It catalyses the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACP1 antibody 22214-1-APDownload protocol
IHC protocol for ACP1 antibody 22214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang
View Article