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验证数据展示

  • WB analysis using 84027-4-RR (same clone as 84027-4-PBS)

    WB analysis using 84027-4-RR (same clone as 84027-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84027-4-RR (same clone as 84027-4-PBS)

    FC experiment of A431 using 84027-4-RR (same clone as 84027-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84027-4-RR

    Affinity and Kinetic Characterization of 84027-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

ACOX3 Recombinant monoclonal antibody, PBS Only

ACOX3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84027-4-PBS

产品说明书

CloneNo. 241172G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

241172G8, ACOX 3, Branched-chain acyl-CoA oxidase, BRCACox, BRCOX

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84027-4-PBS targets ACOX3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10553

Product name: Recombinant human ACOX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 332-624 aa of BC017053

Sequence: TRRQFGPTEEEEIPVLEYPMQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-Coenzyme A oxidase 3, pristanoyl
别名 241172G8, ACOX 3, Branched-chain acyl-CoA oxidase, BRCACox, BRCOX
计算分子量 624 aa, 70 kDa
观测分子量70-78 kDa
GenBank蛋白编号BC017053
基因名称 ACOX3
Gene ID (NCBI) 8310
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO15254
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.

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