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验证数据展示

  • WB analysis using 84027-4-RR

    WB analysis using 84027-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84027-4-RR

    FC experiment of A431 using 84027-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84027-4-RR

    Affinity and Kinetic Characterization of 84027-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

ACOX3 Recombinant monoclonal antibody

ACOX3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84027-4-RR

产品说明书

CloneNo. 241172G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

241172G8, ACOX 3, Branched-chain acyl-CoA oxidase, BRCACox, BRCOX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HL-60 cells, U-251 cells, A549 cells，human placenta tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84027-4-RR targets ACOX3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10553

Product name: Recombinant human ACOX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 332-624 aa of BC017053

Sequence: TRRQFGPTEEEEIPVLEYPMQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-Coenzyme A oxidase 3, pristanoyl
别名 241172G8, ACOX 3, Branched-chain acyl-CoA oxidase, BRCACox, BRCOX
计算分子量 624 aa, 70 kDa
观测分子量70-78 kDa
GenBank蛋白编号BC017053
基因名称 ACOX3
Gene ID (NCBI) 8310
RRIDAB_3671594
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO15254
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.

实验方案

Product Specific Protocols
FC protocol for ACOX3 antibody 84027-4-RRDownload protocol
WB protocol for ACOX3 antibody 84027-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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