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ACOX2 Polyclonal antibody

ACOX2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17571-1-AP

Print datasheet

Synonyms

ACOX2, BRCACOX, BRCOX, THCA CoA oxidase, THCCox

验证数据展示

  • WB analysis using 17571-1-AP

    WB analysis using 17571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17571-1-AP (ACOX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17571-1-AP

    IHC staining of human liver using 17571-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17571-1-AP (ACOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17571-1-AP

    IF Staining of HepG2 using 17571-1-AP

    Immunofluorescent analysis of HepG2 cells, using ACOX2 antibody 17571-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human liver using 17571-1-AP

    IHC staining of human liver using 17571-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17571-1-AP (ACOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse liver using 17571-1-AP

    IP experiment of mouse liver using 17571-1-AP

    IP result of anti-ACOX2 (IP:17571-1-AP, 4ug; Detection:17571-1-AP 1:300) with mouse liver tissue lysate 6000ug.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tisue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17571-1-AP targets ACOX2 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen ACOX2 fusion protein Ag11778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-Coenzyme A oxidase 2, branched chain
Synonyms ACOX2, BRCACOX, BRCOX, THCA CoA oxidase, THCCox
Calculated Molecular Weight 681 aa, 77 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC047700
Gene Symbol ACOX2
Gene ID (NCBI) 8309
RRIDAB_2221803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99424
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACOX2 antibody 17571-1-APDownload protocol
IHC protocol for ACOX2 antibody 17571-1-APDownload protocol
IF protocol for ACOX2 antibody 17571-1-APDownload protocol
IP protocol for ACOX2 antibody 17571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Hypertens Res

Increased expression of acyl-CoA oxidase 2 in the kidney with plasma phytanic acid and altered gut microbiota in spontaneously hypertensive rats.

Authors - Masahiro Okamura
View Article