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验证数据展示

  • WB analysis using RMX00007

    WB analysis using RMX00007

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00007 (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using RMX00007

    IP experiment of HepG2 using RMX00007

    IP result of anti-AOX (IP:RMX00007, 4ug; Detection:RMX00007 1:10000) with HepG2 cells lysate 2000 ug.

  • IHC staining of mouse liver using RMX00007

    IHC staining of mouse liver using RMX00007

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00007 (AOX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using RMX00007

    IF Staining of HepG2 using RMX00007

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AOX antibody (RMX00007) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Multi-rAb™ ACOX1 Multi-Recombinant antibody

ACOX1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00007

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Peroxisomal acyl-CoA oxidase 1, A chain, Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue, HuH-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00007 targets ACOX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 acyl-Coenzyme A oxidase 1, palmitoyl
别名 Peroxisomal acyl-CoA oxidase 1, A chain, Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1
计算分子量 74 kDa
观测分子量70-74kDa, 50 kDa, 22kDa
GenBank蛋白编号BC010425
基因名称 ACOX1
Gene ID (NCBI) 51
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
UNIPROT IDQ15067
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

实验方案

Product Specific Protocols
WB protocol for ACOX1 antibody RMX00007Download protocol
IHC protocol for ACOX1 antibody RMX00007Download protocol
IF protocol for ACOX1 antibody RMX00007Download protocol
IP protocol for ACOX1 antibody RMX00007Download protocol
Standard Protocols
Click here to view our Standard Protocols