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ACOT7 Monoclonal antibody

ACOT7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E9C7

Cat No : 68140-1-Ig

Print datasheet

Synonyms

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

验证数据展示

  • WB analysis using 68140-1-Ig

    WB analysis using 68140-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68140-1-Ig (ACOT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68140-1-Ig targets ACOT7 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Rat, Mouse, Rabbit, Pig
Immunogen ACOT7 fusion protein Ag8975 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acyl-CoA thioesterase 7
Synonyms ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1
Calculated Molecular Weight 370 aa, 40 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC017365
Gene Symbol ACOT7
Gene ID (NCBI) 11332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00154
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acyl-CoA thioesterases (ACOTs) are enzymes that catalyze hydrolysis of fatty acyl-CoAs to free fatty acids and CoA-SH. ACOTs are expressed ubiquitously in prokaryotes and eukaryotes (PMID: 11755680). ACOT7 also known as BACH, CTE-II, ACT, ACH1, BACHa, is one of the most extensively studied ACOTs as a major isoform of the ACOT family (PMID: 17563367). The enzyme is localized to the cytosol and is highly expressed in brain tissue and testis tissue, showing a preference for long-chain acyl-CoA substrates with fatty acid chains of 8-16 carbon atoms (C8-C16) (PMID: 28518146). It has been identified that ACOT7 plays a role in neurotoxic prevention and is a candidate drug target in inflammatory disease (PMID: 17563367, 23459938, 10509865). ACOT7 has 7 isoforms as molecular between 27-40 kDa, it may also form dimer (~70 kDa) (PMID: 20470824).

实验方案

Product Specific Protocols
WB protocol for ACOT7 antibody 68140-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols