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验证数据展示

  • WB analysis using 82122-5-RR (same clone as 82122-5-PBS)

    WB analysis using 82122-5-RR (same clone as 82122-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82122-5-RR (ACOT1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 82122-5-RR (same clone as 82122-5-PBS)

    IHC staining of human liver using 82122-5-RR (same clone as 82122-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82122-5-RR (ACOT1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 82122-5-RR (same clone as 82122-5-PBS)

    IHC staining of human liver using 82122-5-RR (same clone as 82122-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82122-5-RR (ACOT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82122-5-RR (same clone as 82122-5-PBS)

    IF Staining of HepG2 using 82122-5-RR (same clone as 82122-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT1/2 antibody (82122-5-RR, Clone: 251453E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

ACOT1/2 Recombinant monoclonal antibody, PBS Only

ACOT1/2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82122-5-PBS

产品说明书

CloneNo. 251453E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ACOT2, ACOT1, acyl CoA thioesterase 2, Acyl-CoA thioesterase 2, Acyl-coenzyme A thioester hydrolase 2a

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82122-5-PBS targets ACOT1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8093

Product name: Recombinant human ACOT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 222-483 aa of BC006335

Sequence: IVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAMNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLRYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHVMAQVDAWKQLQTFFHKHLGGHEGTIPSKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA thioesterase 2
别名 ACOT2, ACOT1, acyl CoA thioesterase 2, Acyl-CoA thioesterase 2, Acyl-coenzyme A thioester hydrolase 2a
计算分子量 483 aa, 53 kDa
观测分子量 46-53 kDa
GenBank蛋白编号BC006335
基因名称 ACOT2
Gene ID (NCBI) 10965
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49753
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300). ACOT2 shares 98% homology with ACOT1, and this antibody can detect both ACOT1 and ACOT2.

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