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验证数据展示

  • WB analysis using 17871-1-AP (same clone as 17871-1-PBS)

    WB analysis using 17871-1-AP (same clone as 17871-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17871-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 17871-1-AP (same clone as 17871-1-PBS)

    IHC staining of human colon cancer using 17871-1-AP (same clone as 17871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17871-1-AP (ACOT12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17871-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 17871-1-AP (same clone as 17871-1-PBS)

    IF Staining of U2OS using 17871-1-AP (same clone as 17871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT12 antibody (17871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17871-1-PBS in a different storage buffer formulation.

ACOT12 Polyclonal antibody, PBS Only

ACOT12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17871-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Acetyl-coenzyme A thioesterase, ACOT 12, Acyl-CoA thioester hydrolase 12, Acyl-CoA thioesterase 12, Acyl-coenzyme A thioesterase 12

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17871-1-PBS targets ACOT12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag12355

Product name: Recombinant human ACOT12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 206-555 aa of BC075011

Sequence: MAWMETVATISASRLCWAHPFLKSVDMFKFRGPSTVGDRLVFTAIVNNTFQTCVEVGVRVEAFDCQEWAEGRGRHINSAFLIYNAADDKENLITFPRIQPISKDDFRRYRGAIARKRIRLGRKYVISHKEEVPLCIHWDISKQASLSDSNVEALKKLAAKRGWEVTSTVEKIKIYTLEEHDVLSVWVEKHVGSPAHLAYRLLSDFTKRPLWDPHFVSCEVIDWVSEDDQLYHITCPILNDDKPKDLVVLVSRRKPLKDGNTYTVAVKSVILPSVPPSPQYIRSEIICAGFLIHAIDSNSCIVSYFNHMSASILPYFAGNLGGWSKSIEETAASCIQFLENPPDDGFVSTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 12
别名 Acetyl-coenzyme A thioesterase, ACOT 12, Acyl-CoA thioester hydrolase 12, Acyl-CoA thioesterase 12, Acyl-coenzyme A thioesterase 12
计算分子量 555 aa, 62 kDa
观测分子量 70 kDa
GenBank蛋白编号BC075011
基因名称 ACOT12
Gene ID (NCBI) 134526
RRIDAB_3741940
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYK0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACOT12 is an acyl-coenzyme A thioesterase that hydrolyzes acyl-coenzyme A (acyl-CoA) to free fatty acids and coenzyme A, thereby regulating intracellular levels of acyl-CoA and free fatty acids. It plays a key role in lipid metabolism and is mainly expressed in the kidney, liver, and intestine. ACOT12 plays an important role in a variety of diseases, including renal fibrosis, nonalcoholic fatty liver disease (NAFLD), and hepatocellular carcinoma. In renal fibrosis, downregulation of ACOT12 expression leads to lipid accumulation and exacerbates the fibrotic process. Restoration of its expression ameliorates renal fibrosis, suggesting that ACOT12 is a potential therapeutic target.

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