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验证数据展示

  • IF Staining of A431 using 10776-1-AP (same clone as 10776-1-PBS)

    IF Staining of A431 using 10776-1-AP (same clone as 10776-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

    IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

  • WB analysis using 10776-1-AP (same clone as 10776-1-PBS)

    WB analysis using 10776-1-AP (same clone as 10776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

ACOT11 Polyclonal antibody, PBS Only

ACOT11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10776-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10776-1-PBS targets ACOT11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1176

Product name: Recombinant human ACOT11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-221 aa of BC001517

Sequence: MADGEGYRNPTEVQMSQLVLPCHTNQRGELSVGQLLKWIDTTACLSAERHAGCPCVTASMDDIYFEHTISVGQVVNIKAKVNRAFNSSMEVGIQVASEDLCSEKQWNVCKALATFVARREITKVKLKQITPRTEEEKMEHSVAAERRRMRLVYADTIKDLLANCAIQGDLESRDCSRMVPAEKTRVESVELVLPPHANHQGNTFGGQIMAWMENVATIAAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 11
别名
计算分子量68.4 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC001517
基因名称 ACOT11
Gene ID (NCBI) 26027
RRIDAB_2257798
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXI4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).

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