Aconitase 1 Polyclonal antibody
Aconitase 1 Polyclonal Antibody for WB,IP,IF/ICC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,IP,IF/ICC,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, rat liver tissue |
Positive IP detected in | HepG2 cells, mouse liver tissue |
Positive IF detected in | U2OS cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 8 publications below |
IHC | See 1 publications below |
产品信息
12406-1-AP targets Aconitase 1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB,IP,IF/ICC,ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Aconitase 1 fusion protein Ag3083 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aconitase 1, soluble |
Synonyms | ACO1, Aconitase, Aconitase 1, aconitase 1, soluble, ACONS, Citrate hydro lyase, Ferritin repressor protein, IRE BP 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, IRP1 |
Calculated Molecular Weight | 889 aa, 98 kDa |
Observed Molecular Weight | 98 kDa |
GenBank Accession Number | BC018103 |
Gene Symbol | ACO1 |
Gene ID (NCBI) | 48 |
RRID | AB_10642942 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P21399 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
IF protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
IP protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biochim Biophys Acta Mol Basis Dis Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis. | ||
Cell Biosci Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease. | ||
Drug Des Devel Ther Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation. | ||
Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. | ||
JOJ Ophthalmol Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases. | ||
J Exp Clin Cancer Res KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2 |