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验证数据展示

  • WB analysis of HEK-293 using 84100-3-RR (same clone as 84100-3-PBS)

    WB analysis of HEK-293 using 84100-3-RR (same clone as 84100-3-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84100-3-RR (ACMSD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84100-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84100-3-RR (same clone as 84100-3-PBS)

    FC experiment of HepG2 using 84100-3-RR (same clone as 84100-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACMSD Recombinant antibody (84100-3-RR, Clone:241077D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84100-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84100-3-RR

    Affinity and Kinetic Characterization of 84100-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84100-3-RR against Human ACMSD were performed. The affinity constant is 3.49 nM.

ACMSD Recombinant antibody, PBS Only

ACMSD Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84100-3-PBS

产品说明书

CloneNo. 241077D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

EC:4.1.1.45, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, 241077D5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84100-3-PBS targets ACMSD in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ACMSD fusion protein Ag25212 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aminocarboxymuconate semialdehyde decarboxylase
别名 EC:4.1.1.45, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, 241077D5
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC016018
基因名称 ACMSD
Gene ID (NCBI) 130013
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TDX5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACMSD (alpha-amino-beta-carboxy-muconate-semialdehyde decarboxylase ) is the key enzyme that regulates the de novo NAD+ synthesis from tryptophan. ACMSD is highly expressed in the liver, kidney and to a lower degree in brain under physiological conditions. The enzyme is primarily cytosolic and is positioned at a critical branching point in the kynurenine pathway, wherein it influences the fate of the precursor 2-amino-3-carboxymuconic-6-semialdehyde (ACMS).