验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84100-3-RR targets ACMSD in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ACMSD fusion protein Ag25212 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | aminocarboxymuconate semialdehyde decarboxylase |
| 别名 | EC:4.1.1.45, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, 241077D5 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC016018 |
| 基因名称 | ACMSD |
| Gene ID (NCBI) | 130013 |
| RRID | AB_3671665 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8TDX5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACMSD (alpha-amino-beta-carboxy-muconate-semialdehyde decarboxylase ) is the key enzyme that regulates the de novo NAD+ synthesis from tryptophan. ACMSD is highly expressed in the liver, kidney and to a lower degree in brain under physiological conditions. The enzyme is primarily cytosolic and is positioned at a critical branching point in the kynurenine pathway, wherein it influences the fate of the precursor 2-amino-3-carboxymuconic-6-semialdehyde (ACMS).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACMSD antibody 84100-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |