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验证数据展示

  • WB analysis of HEK-293 using 84100-3-RR

    WB analysis of HEK-293 using 84100-3-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84100-3-RR (ACMSD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84100-3-RR

    FC experiment of HepG2 using 84100-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACMSD Recombinant antibody (84100-3-RR, Clone:241077D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84100-3-RR

    Affinity and Kinetic Characterization of 84100-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84100-3-RR against Human ACMSD were performed. The affinity constant is 3.49 nM.

ACMSD Recombinant monoclonal antibody

ACMSD Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84100-3-RR

产品说明书

CloneNo. 241077D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

241077D5, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, EC:4.1.1.45

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84100-3-RR targets ACMSD in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25212

Product name: Recombinant human ACMSD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 47-252 aa of BC016018

Sequence: VSYPRRFVGLGTLPMQAPELAVKEMERCVKELGFPGVQIGTHVNEWDLNAQELFPVYAAAERLKCSLFVHPWDMQMDGRMAKYWLPWLVGMPAETTIAICSMIMGGVFEKFPKLKVCFAHGGGAFPFTVGRISHGFSMRPDLCAQDNPMNPKKYLGSFYTDALVHDPLSLKLLTDVIGKDKVILGTDYPFPLGELEPGKLIESMEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aminocarboxymuconate semialdehyde decarboxylase
别名 241077D5, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, EC:4.1.1.45
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC016018
基因名称 ACMSD
Gene ID (NCBI) 130013
RRIDAB_3671665
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TDX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACMSD (alpha-amino-beta-carboxy-muconate-semialdehyde decarboxylase ) is the key enzyme that regulates the de novo NAD+ synthesis from tryptophan. ACMSD is highly expressed in the liver, kidney and to a lower degree in brain under physiological conditions. The enzyme is primarily cytosolic and is positioned at a critical branching point in the kynurenine pathway, wherein it influences the fate of the precursor 2-amino-3-carboxymuconic-6-semialdehyde (ACMS).

实验方案

Product Specific Protocols
WB protocol for ACMSD antibody 84100-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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