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验证数据展示

  • WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

    WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

    IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACLY antibody included
  • IF Staining of A549 using 67166-1-Ig (same clone as 67166-1-PBS)

    IF Staining of A549 using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (67166-1-Ig, Clone: 1E10D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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ACLY Monoclonal antibody, PBS Only

ACLY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67166-1-PBS

产品说明书

CloneNo. 1E10D5

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ATP citrate lyase, 1E10D5, ACL, ATP-citrate, ATP-citrate synthase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy ACLY Ready-To-Use IHC Kit. ACLY primary antibody included.

产品信息

67166-1-PBS targets ACLY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7709

Product name: Recombinant human ACLY protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 751-1101 aa of BC006195

Sequence: MFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP citrate lyase
别名 ATP citrate lyase, 1E10D5, ACL, ATP-citrate, ATP-citrate synthase
计算分子量 121 kDa
观测分子量 120 kDa
GenBank蛋白编号BC006195
基因名称 ACLY
Gene ID (NCBI) 47
RRIDAB_2882462
偶联类型 Unconjugated
形式Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP53396
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

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