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ACLY Polyclonal antibody

ACLY Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat No : 15421-1-AP

Print datasheet

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

验证数据展示

WB analysis using 15421-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15421-1-AP
WB result of ACLY antibody (15421-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACLY transfected A549 cells.
IF Staining of HepG2 using 15421-1-AP
Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
WB analysis using 15421-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15421-1-AP
IP result of anti-ACLY (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:8000) with HeLa cells lysate 1320 ug.
FC experiment of HepG2 using 15421-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ACLY (15421-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of human prostate cancer using 15421-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 15421-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 15421-1-AP

Size

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经过测试的应用

Positive WB detected in	L02 cells, A549 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 14 publications below
WB	See 59 publications below
IHC	See 5 publications below
IF	See 7 publications below
IP	See 3 publications below
CoIP	See 3 publications below

产品信息

15421-1-AP targets ACLY in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IP, IF, IHC, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Immunogen	ACLY fusion protein Ag7709 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ATP citrate lyase
Synonyms	ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP
Calculated Molecular Weight	121 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC006195
Gene Symbol	ACLY
Gene ID (NCBI)	47
RRID	AB_2223741
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53396
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACLY antibody 15421-1-AP	Download protocol
IHC protocol for ACLY antibody 15421-1-AP	Download protocol
IF protocol for ACLY antibody 15421-1-AP	Download protocol
IP protocol for ACLY antibody 15421-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nature Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate. Authors - Steven Zhao View Article
mouse	WB,IF	Nature Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory. Authors - Philipp Mews View Article
human,mouse	WB	Cell Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals. Authors - Xiaojing Liu KO Validated View Article
mouse	WB	Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate Authors - Peng Zhou View Article
mouse	WB	Mol Cell Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation Authors - Sophie Trefely KO Validated View Article
human	WB	Mol Cell Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination. Authors - Sharanya Sivanand KD Validated View Article

ACLY Polyclonal antibody

ACLY Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis using 15421-1-AP

WB analysis of A549 using 15421-1-AP

IF Staining of HepG2 using 15421-1-AP

WB analysis using 15421-1-AP

IP experiment of HeLa using 15421-1-AP

FC experiment of HepG2 using 15421-1-AP

IHC staining of human prostate cancer using 15421-1-AP

IHC staining of human prostate cancer using 15421-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.

相关产品

Flow Cytometry Perm Buffer (10X)