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验证数据展示

  • WB analysis using 31518-1-AP

    WB analysis using 31518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31518-1-AP (ACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31518-1-AP

    IHC staining of mouse testis using 31518-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31518-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACE2 antibody included

ACE2 Polyclonal antibody

ACE2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31518-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ACE related carboxypeptidase, ACEH, ACE-related carboxypeptidase, Angiotensin converting enzyme 2, Angiotensin-converting enzyme 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse small intestine tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ACE2 Ready-To-Use IHC Kit. ACE2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31518-1-AP targets ACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg0074

Product name: Recombinant Human ACE2 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: N-6*His

Domain: 18-740 aa of BC048094

Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
别名 ACE related carboxypeptidase, ACEH, ACE-related carboxypeptidase, Angiotensin converting enzyme 2, Angiotensin-converting enzyme 2
计算分子量 805 aa, 92 kDa
观测分子量120 kDa
GenBank蛋白编号BC048094
基因名称 ACE2
Gene ID (NCBI) 59272
RRIDAB_3670016
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BYF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiotensin-converting enzyme 2 (ACE2), a homolog of angiotensin-converting enzyme 1 (ACE1), was identified in 2000. ACE2 provides protection against several chronic diseases, including cardiovascular diseases, lung injury, and diabetes. Besides chronic diseases, ACE2 plays a crucial role in severe acute respiratory syndrome coronavirus (SARS-CoV) infection and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (hereafter named COVID-19). ACE2 is the host receptor for SARS-CoV and SARS-CoV-2. (PMID: 37608279)

实验方案

Product Specific Protocols
IHC protocol for ACE2 antibody 31518-1-APDownload protocol
WB protocol for ACE2 antibody 31518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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