验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | rat lung tissue, human lung tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24743-1-AP targets ACE in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 |
CatNo: Ag20654 Product name: Recombinant human ACE protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 390-739 aa of BC036375 Sequence: FYNGKDFRIKQCTTVNLEDLVVAHHEMGHIQYFMQYKDLPVALREGANPGFHEAIGDVLALSVSTPKHLHSLNLLSSEGGSDEHDINFLMKMALDKIAFIPFSYLVDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRTQGDFDPGAKFHIPSSVPYIRYFVSFIIQFQFHEALCQAAGHTGPLHKCDIYQSKEAGQRLATAMKLGFSRPWPEAMQLITGQPNMSASAMLSYFKPLLDWLRTENELHGEKLGWPQYNWTPNSDDFYNETETKIFLQFYDQTGIWDHGAPHLLPPSQARGTREAPVYMSKRAASSGPPGSPKLPPAAQGHGEVPVHDLLWHPGPPV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 |
| 别名 | ACE1, Angiotensin converting enzyme, Angiotensin-converting enzyme, Angiotensin-converting enzyme, soluble form, DCP |
| 计算分子量 | 150 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC036375 |
| 基因名称 | ACE |
| Gene ID (NCBI) | 1636 |
| RRID | AB_2879701 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12821 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ACE antibody 24743-1-AP | Download protocol |
| IHC protocol for ACE antibody 24743-1-AP | Download protocol |
| WB protocol for ACE antibody 24743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction. | ||
J Med Chem Hypoxia Activated Nitric Oxide Donor Compounds for the Prevention and Treatment of Myocardial Hypoxia-Induced Injury | ||
Diabetol Metab Syndr Celastrol attenuates streptozotocin-induced diabetic cardiomyopathy in mice by inhibiting the ACE / Ang II / AGTR1 signaling pathway | ||
Am J Physiol Renal Physiol Adequate salt intake is essential for candesartan-treated rats to maintain renal function | ||
ACS Omega Azelaic Acid Regulates the Renin-Angiotensin System and Improves Colitis Based on Network Pharmacology and Experimentation |
