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验证数据展示

  • WB analysis of MDA-MB-453s using 11491-1-AP

    WB analysis of MDA-MB-453s using 11491-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11491-1-AP

    IHC staining of human colon cancer using 11491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11491-1-AP (PHACS antibody) at dilution of 1:50 (under 40x lens).

PHACS Polyclonal antibody

PHACS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11491-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ACC synthase like protein 1, ACCS, ACS, PHACS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453s cells, HeLa cells, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11491-1-AP targets PHACS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PHACS fusion protein Ag2056 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional)
别名 ACC synthase like protein 1, ACCS, ACS, PHACS
计算分子量 501 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC020197
基因名称 PHACS
Gene ID (NCBI) 84680
RRIDAB_2222681
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QU6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACCS(1-aminocyclopropane-1-carboxylate synthase-like protein 1) is also named as PHACS and belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. The deduced 501-amino acid protein has a calculated molecular mass of 58 kD. It contains overlapping aminotransferase I and beta-eliminating lyase domains, and it shares structural similarity with aspartate aminotransferase, tyrosine aminotransferase, and enzymes that catalyze beta-elimination reactions on amino acids.

实验方案

Product Specific Protocols
WB protocol for PHACS antibody 11491-1-APDownload protocol
IHC protocol for PHACS antibody 11491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols