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验证数据展示

  • IHC staining of mouse testis using 14096-1-AP

    IHC staining of mouse testis using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • WB analysis using 14096-1-AP

    WB analysis using 14096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14096-1-AP

    IF Staining of HeLa using 14096-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14096-1-AP (ACBD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse ovary using 14096-1-AP

    IHC staining of mouse ovary using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of mouse ovary using 14096-1-AP

    IHC staining of mouse ovary using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of human placenta using 14096-1-AP

    IHC staining of human placenta using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of human stomach cancer using 14096-1-AP

    IHC staining of human stomach cancer using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of mouse cerebellum using 14096-1-AP

    IHC staining of mouse cerebellum using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of mouse stomach using 14096-1-AP

    IHC staining of mouse stomach using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of rat ovary using 14096-1-AP

    IHC staining of rat ovary using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of rat stomach using 14096-1-AP

    IHC staining of rat stomach using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of mouse stomach using 14096-1-AP

    IHC staining of mouse stomach using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of rat ovary using 14096-1-AP

    IHC staining of rat ovary using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included
  • IHC staining of rat stomach using 14096-1-AP

    IHC staining of rat stomach using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACBD3 antibody included

ACBD3 Polyclonal antibody

ACBD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14096-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

Acyl-CoA-binding domain-containing protein 3, GCP60, GOCAP1, Golgi complex-associated protein 1, Golgi phosphoprotein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, mouse ovary tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue, rat stomach tissue, human stomach cancer tissue, human placenta tissue, mouse ovary tissue, mouse cerebellum tissue, rat ovary tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ACBD3 Ready-To-Use IHC Kit. ACBD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14096-1-AP targets ACBD3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag5228

Product name: Recombinant human ACBD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 229-528 aa of BC060792

Sequence: RRRIEEERLRLEQQKQQIMAALNSQTAVQFQQYAAQQYPGNYEQQQILIRQLQEQHYQQYMQQLYQVQLAQQQAALQKQQEVVVAGSSLPTSSKVNATVPSNMMSVNGQAKTHTDSSEKELEPEAAEEALENGPKESLPVIAAPSMWTRPQIKDFKEKIQQDADSVITVGRGEVVTVRVPTHEEGSYLFWEFATDNYDIGFGVYFEWTDSPNTAVSVHVSESSDDDEEEEENIGCEEKAKKNANRPLLDEIVPVYRRDCHEEVYAGSHQYPGRGVYLLKFDNSYSLWRSKSVYYRVYYTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-Coenzyme A binding domain containing 3
别名 Acyl-CoA-binding domain-containing protein 3, GCP60, GOCAP1, Golgi complex-associated protein 1, Golgi phosphoprotein 1
计算分子量 528 aa, 61 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC060792
基因名称 ACBD3
Gene ID (NCBI) 64746
RRIDAB_2220062
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3P7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ACBD3 antibody 14096-1-APDownload protocol
IHC protocol for ACBD3 antibody 14096-1-APDownload protocol
WB protocol for ACBD3 antibody 14096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Transl Psychiatry

Critical role of translocator protein (TSPO) in neuronal mitochondrial dysfunction and mental stress-exacerbated ischemic injury following stroke.

Authors - Yuequan Zhu
View Article

Int Immunopharmacol

HO-1 alleviates lipopolysaccharide-induced acute lung injury in mice by downregulating TFE3 expression and nuclear translocation and suppressing Golgi stress response.

Authors - Xiangyun Li
View Article
humanIF

J Bacteriol

The Chlamydia pneumoniae inclusion membrane protein Cpn0308 interacts with host protein ACBD3

Authors - Liang Ma
View Article
humanWB

Cell Biol Int

Golgi protein ACBD3 downregulation sensitizes cells to ferroptosis

Authors - Ying Qian
  • KD Validated
View Article

Infect Immun

Chlamydia pneumoniae facilitates its development by recruiting PI4P to inclusion bodies via the Cpn0308-ACBD3-PI4KB pathway.

Authors - Xiaonan Feng
View Article
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