验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, Jurkat cells |
| Positive IP detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14029-1-AP targets ACAP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, canine |
| 免疫原 |
CatNo: Ag5190 Product name: Recombinant human ACAP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 479-778 aa of BC060767 Sequence: YEANVEKMGIKKPQPGQRQEKEAYIRAKYVERKFVDKYSISLSPPEQQKKFVSKSSEEKRLSISKFGPGDQVRASAQSSVRSNDSEIQQSSDDGRESLPSTVSANSLYEPEGERQDSSMFLDSKHLNPGLQLYRASYEKNLPKMAEALAHGADVNWANSEENKATPLIQAVLGGSLVTCEFLLQNGANVNQRDVQGRGPLHHATVLGHTGQVCLFLKRGANQHATDEEGKDPLSIAVEAANADIVTLLRLARMNEEMRESEGLYGQPGDETYQDIFRDFSQMASNNPEKLNRFQQDSQKF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 |
| 别名 | ACAP2, Centaurin beta 2, CENTB2, CNT B2, KIAA0041 |
| 计算分子量 | 778 aa, 88 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC060767 |
| 基因名称 | ACAP2 |
| Gene ID (NCBI) | 23527 |
| RRID | AB_2219956 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15057 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ACAP2 antibody 14029-1-AP | Download protocol |
| WB protocol for ACAP2 antibody 14029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis. | ||
Cell Death Dis Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.
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