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验证数据展示

  • WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

    IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB result of ACAP1 antibody (66596-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ACAP1 Monoclonal antibody, PBS Only

ACAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66596-1-PBS

产品说明书

CloneNo. 1B4C9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66596-1-PBS targets ACAP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14254

Product name: Recombinant human ACAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-251 aa of BC018543

Sequence: SIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
别名 Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
计算分子量 740 aa, 82 kDa
观测分子量 81 kDa
GenBank蛋白编号BC018543
基因名称 ACAP1
Gene ID (NCBI) 9744
RRIDAB_2881956
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15027
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.