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验证数据展示

  • IHC staining of human lung cancer using 68350-1-Ig (same clone as 68350-1-PBS)

    IHC staining of human lung cancer using 68350-1-Ig (same clone as 68350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68350-1-Ig (ACAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACAN antibody included
  • IF Staining of HEK-293 using 68350-1-Ig (same clone as 68350-1-PBS)

    IF Staining of HEK-293 using 68350-1-Ig (same clone as 68350-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ACAN antibody (68350-1-Ig, Clone: 1G11B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

Aggrecan Monoclonal antibody, PBS Only

Aggrecan Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 68350-1-PBS

产品说明书

CloneNo. 1G11B3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

ACAN, 1G11B3, Aggrecan core protein 2, Aggrecan 1, AGCAN

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ACAN Ready-To-Use IHC Kit. ACAN primary antibody included.

产品信息

68350-1-PBS targets Aggrecan in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4985

Product name: Recombinant human ACAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC036445

Sequence: MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 aggrecan
别名 ACAN, 1G11B3, Aggrecan core protein 2, Aggrecan 1, AGCAN
计算分子量 250 kDa
GenBank蛋白编号BC036445
基因名称 ACAN
Gene ID (NCBI) 176
RRIDAB_3085073
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16112
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aggrecan (ACAN), also named CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of the extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

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