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验证数据展示

  • IHC staining of human lung cancer using 68350-1-Ig (same clone as 68350-1-PBS)

    IHC staining of human lung cancer using 68350-1-Ig (same clone as 68350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68350-1-Ig (ACAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACAN antibody included
  • IF Staining of HEK-293 using 68350-1-Ig (same clone as 68350-1-PBS)

    IF Staining of HEK-293 using 68350-1-Ig (same clone as 68350-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ACAN antibody (68350-1-Ig, Clone: 1G11B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

Aggrecan Monoclonal antibody, PBS Only

Aggrecan Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 68350-1-PBS

产品说明书

CloneNo. 1G11B3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

ACAN, 1G11B3, Aggrecan core protein 2, Aggrecan 1, AGCAN

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ACAN Ready-To-Use IHC Kit. ACAN primary antibody included.

产品信息

68350-1-PBS targets Aggrecan in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4985

Product name: Recombinant human ACAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC036445

Sequence: MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 aggrecan
别名 ACAN, 1G11B3, Aggrecan core protein 2, Aggrecan 1, AGCAN
计算分子量 250 kDa
GenBank蛋白编号BC036445
基因名称 ACAN
Gene ID (NCBI) 176
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16112
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aggrecan (ACAN), also named CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of the extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

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