Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, FC (Intra), ELISA
Cat No. 13880-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

IHC, FC (Intra), ELISA and More (2)

ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive IHC detected inhuman lung tissue, human cartilage tissue, human lung cancer tissue, mouse lung tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inATDC-5 cells
Planning an IHC experiment? We recommend our IHCeasy ACAN Ready-To-Use IHC Kit. ACAN primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13880-1-AP targets Aggrecan in IHC, IF, FC (Intra), ELISA, IHC-IF applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, FC (Intra), ELISA Application Description
文献引用应用IHC, IF, IHC-IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, canine, zebrafish
免疫原 Aggrecan fusion protein Ag4985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aggrecan
别名 ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN
计算分子量 250 kDa
GenBank蛋白编号BC036445
基因名称 ACAN
Gene ID (NCBI) 176
RRIDAB_2722780
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

实验方案

Product Specific Protocols
IHC protocol for Aggrecan antibody 13880-1-APDownload protocol
FC protocol for Aggrecan antibody 13880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
human,mouseIF

Cell Stem Cell

A human organoid drug screen identifies α2-adrenergic receptor signaling as a therapeutic target for cartilage regeneration

Authors - Xiaocui Wei
human

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
human

J Extracell Vesicles

miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.

Authors - Hongxing Hu
ratIF

Biomaterials

Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration

Authors - Lei Liu
mouseIHC,IF

Bone Res

Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome

Authors - Yanqiu Wang
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