验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, HepG2 cells, HEK-293 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13122-1-AP targets ACADSB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | ACADSB fusion protein Ag3851 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | acyl-Coenzyme A dehydrogenase, short/branched chain |
别名 | 2 MEBCAD, ACAD7, ACADSB, SBCAD |
计算分子量 | 432 aa, 47 kDa |
观测分子量 | 45-47 kDa |
GenBank蛋白编号 | BC013756 |
基因名称 | ACADSB |
Gene ID (NCBI) | 36 |
RRID | AB_10638904 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P45954 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ACADSB antibody 13122-1-AP | Download protocol |
IHC protocol for ACADSB antibody 13122-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery | ||
Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. | ||
Front Oncol Decreased Expression of ACADSB Predicts Poor Prognosis in Clear Cell Renal Cell Carcinoma. | ||
Cell Biol Int ACADSB regulates ferroptosis and affects the migration, invasion, and proliferation of colorectal cancer cells. | ||
Nat Commun AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis | ||
Phytother Res Icariside II prevents kidney fibrosis development in chronic kidney disease by promoting fatty acid oxidation |