• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ACADM Monoclonal antibody

ACADM Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G6

Cat No : 67742-1-Ig

Print datasheet

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

验证数据展示

  • WB analysis using 67742-1-Ig

    WB analysis using 67742-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of rat liver using 67742-1-Ig

    WB analysis of rat liver using 67742-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67742-1-Ig

    IHC staining of human liver using 67742-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67742-1-Ig

    IHC staining of human liver using 67742-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 67742-1-Ig

    IF Staining of human liver cancer using 67742-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HSC-T6 using 67742-1-Ig

    WB analysis of HSC-T6 using 67742-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, HSC-T6 cells, rat liver tissue, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67742-1-Ig targets ACADM in WB,IHC,IF,ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, rat, pig
Cited Reactivityrat
Immunogen ACADM fusion protein Ag30620 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Synonyms ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH
Calculated Molecular Weight 421 aa, 47 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC005377
Gene Symbol ACADM
Gene ID (NCBI) 34
RRIDAB_2918511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11310
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

实验方案

Product Specific Protocols
WB protocol for ACADM antibody 67742-1-IgDownload protocol
IHC protocol for ACADM antibody 67742-1-IgDownload protocol
IF protocol for ACADM antibody 67742-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int J Mol Sci

High-Dose Fenofibrate Stimulates Multiple Cellular Stress Pathways in the Kidney of Old Rats

Authors - Agata Wrońska
View Article