• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86161-3-RR

    WB analysis using 86161-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86161-3-RR (ACAA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86161-3-RR

    IF Staining of HepG2 using 86161-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAA2 antibody (86161-3-RR, Clone: 250814D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ACAA2 Recombinant antibody

ACAA2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86161-3-RR

产品说明书

CloneNo. 250814D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Ramos cells, Caco-2 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACAA2 Ready-To-Use IHC Kit. ACAA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86161-3-RR targets ACAA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACAA2 fusion protein Ag1609 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acetyl-Coenzyme A acyltransferase 2
别名 3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial
计算分子量 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC001918
基因名称 ACAA2
Gene ID (NCBI) 10449
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42765
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACAA2 antibody 86161-3-RRDownload protocol
IF protocol for ACAA2 antibody 86161-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols