验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human colon tissue, human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ACAA2 Ready-To-Use IHC Kit. ACAA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11111-1-AP targets ACAA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, sheep |
| 免疫原 |
CatNo: Ag1609 Product name: Recombinant human ACAA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 98-397 aa of BC001918 Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acetyl-Coenzyme A acyltransferase 2 |
| 别名 | 3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC001918 |
| 基因名称 | ACAA2 |
| Gene ID (NCBI) | 10449 |
| RRID | AB_2219389 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42765 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACAA2 antibody 11111-1-AP | Download protocol |
| IHC protocol for ACAA2 antibody 11111-1-AP | Download protocol |
| IF protocol for ACAA2 antibody 11111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition | ||
Nat Chem Biol The molecular glue CLEO4-88 inhibits the ACAA1 thiolase by induced binding to GID4. | ||
J Pharm Anal Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice. | ||
EBioMedicine PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules. | ||
Phytother Res Icariside II prevents kidney fibrosis development in chronic kidney disease by promoting fatty acid oxidation | ||
Free Radic Biol Med Low-intensity pulsed ultrasound ameliorates pancreatic injury and glucose metabolism in type 2 diabetic mice: regulatory role of the oxidative phosphorylation pathway. |
