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STARS/ABRA Polyclonal antibody

STARS/ABRA Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 22673-1-AP

Print datasheet

Synonyms

ABRA, STARS

验证数据展示

  • WB analysis of mouse heart using 22673-1-AP

    WB analysis of mouse heart using 22673-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 22673-1-AP (STARS/ABRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 22673-1-AP

    IHC staining of human heart using 22673-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22673-1-AP (STARS/ABRA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 22673-1-AP

    IHC staining of human heart using 22673-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22673-1-AP (STARS/ABRA antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 22673-1-AP

    IF Staining of HepG2 using 22673-1-AP

    Immunofluorescent analysis of HepG2 cells using 22673-1-AP (STARS/ABRA antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22673-1-AP is STARS/ABRA Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen STARS/ABRA fusion protein Ag18456 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin-binding Rho activating protein
Synonyms ABRA, STARS
Calculated Molecular Weight 381 aa, 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC105103
Gene Symbol ABRA
Gene ID (NCBI) 137735
RRIDAB_11182712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STARS/ABRA antibody 22673-1-APDownload protocol
IHC protocol for STARS/ABRA antibody 22673-1-APDownload protocol
IF protocol for STARS/ABRA antibody 22673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Development

HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.

Authors - Meng Xia
View Article
mouseWB

Behav Brain Res

Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice

Authors - Yexiang Chen
View Article
human,mouseWB,IF

Am J Pathol

Different mouse models of nemaline myopathy harboring Acta1 mutations display differing abnormalities related to mitochondrial biology

Authors - Jennifer A Tinklenberg
View Article