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验证数据展示

  • WB analysis using 16227-1-AP (same clone as 16227-1-PBS)

    WB analysis using 16227-1-AP (same clone as 16227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16227-1-AP (ABI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16227-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 16227-1-AP (same clone as 16227-1-PBS)

    IHC staining of mouse heart using 16227-1-AP (same clone as 16227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16227-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 16227-1-AP (same clone as 16227-1-PBS)

    IHC staining of mouse heart using 16227-1-AP (same clone as 16227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16227-1-PBS in a different storage buffer formulation.

ABI3 Polyclonal antibody, PBS Only

ABI3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16227-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

SSH3BP3, New molecule including SH3, Nesh, ABI gene family member 3, ABI family, member 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16227-1-PBS targets ABI3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8164

Product name: Recombinant human ABI3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-366 aa of BC007780

Sequence: MAELQQLQEFEIPTGREALRGNHSALLRVADYCEDNYVQATDKRKALEETMAFTTQALASVAYQVGNLAGHTLRMLDLQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPPGQKVIAPENLPPLTPYCRRPLNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATPASATLGRPPRIPEPVHLPVVPDGRLSAASSASSLASAGSAEGVGGAPTPKGQAAPPAPPLPSSLDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDLPPPPPLDGDELGLPPPPPGFGPDEPSWVPASYLEKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSEGTGFFPGNYVEPSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ABI family, member 3
别名 SSH3BP3, New molecule including SH3, Nesh, ABI gene family member 3, ABI family, member 3
计算分子量 366 aa, 39 kDa
观测分子量50 kDa
GenBank蛋白编号BC007780
基因名称 ABI3
Gene ID (NCBI) 51225
RRIDAB_3741879
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2A4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABI family member 3 belongs to the ABL Interactors (ABI) family of proteins, which also includes ABI1 and ABI2. ABI proteins are a class of cytoplasmic molecular junction proteins originally identified as binding chaperones for Abl kinase. The ABI3 gene is thought to be an important regulator of microglia during Alzheimer's disease (AD), and its S209F variant increases the risk of developing AD. Moreover, ABI3 plays an important role in suppressing tumor growth and progression.

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