验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse heart tissue, mouse uterus tissue, mouse testis tissue, mouse brain tissue, HeLa cells, K-562 cells, SH-SY5Y cells |
| Positive IHC detected in | human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy® ABI2 Ready-To-Use IHC Kit. ABI2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| CoIP | See 1 publications below |
产品信息
14890-1-AP targets ABI2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6679 Product name: Recombinant human ABI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC001439 Sequence: MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | abl interactor 2 |
| 别名 | Abelson interactor 2, ABI 2, ABI2, ABI2B, Abl binding protein 3, abl interactor 2, AblBP3, AIP 1, Arg binding protein 1, ArgBP1, argBPIA, argBPIB, SSH3BP2 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 52-56 kDa |
| GenBank蛋白编号 | BC001439 |
| 基因名称 | ABI2 |
| Gene ID (NCBI) | 10152 |
| RRID | AB_2288997 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYB9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ABI2 antibody 14890-1-AP | Download protocol |
| WB protocol for ABI2 antibody 14890-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis | ||
Nat Commun hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS) | ||
Nat Cell Biol Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis |
