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验证数据展示

  • WB analysis of L02 using 20952-1-AP

    WB analysis of L02 using 20952-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 20952-1-AP

    IHC staining of human prostate hyperplasia using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ABHD14B antibody included
  • IHC staining of human prostate hyperplasia using 20952-1-AP

    IHC staining of human prostate hyperplasia using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ABHD14B antibody included
  • IF Staining of HepG2 using 20952-1-AP

    IF Staining of HepG2 using 20952-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ABHD14B antibody (20952-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ABHD14B Polyclonal antibody

ABHD14B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20952-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Abhydrolase domain-containing protein 14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, CIB, EC:2.3.1.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, mouse small intestine tissue
Positive IHC detected inhuman prostate hyperplasia tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ABHD14B Ready-To-Use IHC Kit. ABHD14B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20952-1-AP targets ABHD14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15097

Product name: Recombinant human ABHD14B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-210 aa of BC007234

Sequence: MAASVEQREGTIQVQGQALFFREALPGSGQARFSVLLLHGIRFSSETWQNLGTLHRLAQAGYRAVAIDLPGLGHSKEAAAPAPIGELAPGSFLAAVVDALELGPPVVISPSLSGMYSLPFLTAPGSQLPGFVPVAPICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFLQGLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 14B
别名 Abhydrolase domain-containing protein 14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, CIB, EC:2.3.1.-
计算分子量 210 aa, 22 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC007234
基因名称 ABHD14B
Gene ID (NCBI) 84836
RRIDAB_10733758
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IU4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABHD14B possessed the canonical ABHD fold, an invariant catalytic triad (Ser-His-Asp), and based on its protein sequence, was subsequently categorized as an outlying member of the metabolic serine hydrolase family. ABHD14B was able to transfer an acetyl group from post translationally modified protein lysine residue to coenzyme-A (CoA), thus making the biologically important acetyl-CoA.

实验方案

Product Specific Protocols
IF protocol for ABHD14B antibody 20952-1-APDownload protocol
IHC protocol for ABHD14B antibody 20952-1-APDownload protocol
WB protocol for ABHD14B antibody 20952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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