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验证数据展示

  • WB analysis of mouse kidney using 19419-1-AP

    WB analysis of mouse kidney using 19419-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19419-1-AP (ABHD14A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 19419-1-AP

    IP experiment of mouse kidney using 19419-1-AP

    IP result of anti-ABHD14A (IP:19419-1-AP, 4ug; Detection:19419-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

  • IF Staining of HeLa using 19419-1-AP

    IF Staining of HeLa using 19419-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19419-1-AP (ABHD14A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 19419-1-AP

    IF Staining of HeLa using 19419-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABHD14A antibody (19419-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ABHD14A Polyclonal antibody

ABHD14A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 19419-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Protein ABHD14A, EC:3.-.-.-, DORZ1, Alpha/beta hydrolase domain-containing protein 14A, Abhydrolase domain-containing protein 14A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19419-1-AP targets ABHD14A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13744

Product name: Recombinant human ABHD14A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 56-271 aa of BC002571

Sequence: PEQTSCLWGDPNVTVLAGLTPGNSPIFYREVLPLNQAHRVEVVLLHGKAFNSHTWEQLGTLQLLSQRGYRAVALDLPGFGNSAPSKEASTEAGRAALLERALRDLEVQNAVLVSPSLSGHYALPFLMRGHHQLHGFVPIAPTSTQNYTQEQFWAVKTPTLILYGELDHILARESLRQLRHLPNHSVVKLRNAGHACYLHKPQDFHLVLLAFLDHLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 14A
别名 Protein ABHD14A, EC:3.-.-.-, DORZ1, Alpha/beta hydrolase domain-containing protein 14A, Abhydrolase domain-containing protein 14A
计算分子量 271 aa, 30 kDa
观测分子量 30-32 kDa
GenBank蛋白编号BC002571
基因名称 ABHD14A
Gene ID (NCBI) 25864
RRIDAB_10642140
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ABHD14A antibody 19419-1-APDownload protocol
IP protocol for ABHD14A antibody 19419-1-APDownload protocol
WB protocol for ABHD14A antibody 19419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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