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验证数据展示

  • WB analysis of mouse skin using 20623-1-AP

    WB analysis of mouse skin using 20623-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20623-1-AP (ABHD12B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20623-1-AP

    IHC staining of human skin using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 20623-1-AP

    IHC staining of human skin using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 40x lens).

ABHD12B Polyclonal antibody

ABHD12B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20623-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ABHD12B, BEM46L3, c14_5314, C14orf29

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, mouse kidney tissue
Positive IHC detected inhuman skin tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20623-1-AP targets ABHD12B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14426

Product name: Recombinant human ABHD12B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-255 aa of BC034603

Sequence: MLGIWHTVPSCRGEDAKGKDCCWYEAALRDGNPIIVYLHGSAEHRAASHRLKLVKVLSDGGFHVLSVDYRGFGDSTGKPTEEGLTTDAICVYEWTKARSGITPVCLWGHSLGTGVATNAAKVLEEKGCPVDAIVLEAPFTNMWVASINYPLLKIYRNIPGFLRTLMDALRKDKIIFPNDENVKFLSSPLLILHGEDDRTVPLEYGKKLYEIARNAYRNKERVKMVIFPPGFQHNLLCKSPTLLITVRDFLSKQWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 12B
别名 ABHD12B, BEM46L3, c14_5314, C14orf29
计算分子量 362 aa, 41 kDa
观测分子量 41 kDa, 29 kDa
GenBank蛋白编号BC034603
基因名称 ABHD12B
Gene ID (NCBI) 145447
RRIDAB_10693623
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z5M8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ABHD12B antibody 20623-1-APDownload protocol
WB protocol for ABHD12B antibody 20623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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