验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22660-1-AP targets ABHD12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18522 Product name: Recombinant human ABHD12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-404 aa of BC014049 Sequence: FLIKLCPGIQAKLIFLNFVRVPYFIDLKKPQDQGLNHTCNYYLQPEEDVTIGVWHTVPAVWWKNAQGKDQMWYEDALASSHPIILYLHGNAGTRGGDHRVELYKVLSSLGYHVVTFDYRGWGDSVGTPSERGMTYDALHVFDWIKARSGDNPVYIWGHSLGTGVATNLVRRLCERETPPDALILESPFTNIREEAKSHPFSVIYRYFPGFDWFFLDPITSSGIKFANDENVKHISCPLLILHAEDDPVVPFQLGRKLYSIAAPARSFRDFKVQFVPFHSDLGYRHKYIYKSPELPRILRPQQGPGSSPDPSMWSELV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | abhydrolase domain containing 12 |
| 别名 | ABHD12A, BEM46L2, C20orf22 |
| 计算分子量 | 404 aa, 46 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC014049 |
| 基因名称 | ABHD12 |
| Gene ID (NCBI) | 26090 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N2K0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABHD12 is a recently identified post-genomic protein with poorly defined physiological function. Its potential role as a brain 2-AG hydrolase was revealed using ABPP with mouse brain proteome and it was estimated that at the bulk brain level. ABHD12 is a major brain lyso-PS lipase, implicating lyso-PSs in the neurological disease PHARC, which is caused by null mutations in the ABHD12 gene (PMID: 21418147, 25580854).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD12 antibody 22660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
