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验证数据展示

  • WB analysis using 25355-1-AP

    WB analysis using 25355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25355-1-AP (ABHD11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 25355-1-AP

    IF Staining of PC-3 using 25355-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABHD11 antibody (25355-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ABHD11 Polyclonal antibody

ABHD11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25355-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

sn-1-specific diacylglycerol lipase ABHD11, PP1226, EC:3.1.1.116, Alpha/beta hydrolase domain-containing protein 11, Abhydrolase domain-containing protein 11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, PC-3 cells, human placenta tissue
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25355-1-AP targets ABHD11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18318

Product name: Recombinant human ABHD11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC067750

Sequence: MRAGQQLASMLRWTRAWRLPREGLGPHGPSFARVPVAPSSSSGGRGGAEPRPLPLSYRLLDGEAALPAVVFLHGLFGSKTNFNSIAKILAQQTGRRVLTVDARNHGDSPHSPDMSYEIMSQDLQDLLPQLGLVPCVVVGHSMGGKTAMLLALQRPELVERLIAVDISPVESTGVSHFATYVAAMRAINIADELPRSRARKLADEQLSSVIQDMAVRQHLLTNLVEVDGRFVWRVNLDALTQHLDKILAFPQRQESYLGPTLFLLGGNSQFVHPSHHPEIMRLFPRAQMQTVPNAGHWIHADRPQDFIAAIRGFLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 11
别名 sn-1-specific diacylglycerol lipase ABHD11, PP1226, EC:3.1.1.116, Alpha/beta hydrolase domain-containing protein 11, Abhydrolase domain-containing protein 11
计算分子量 315 aa, 35 kDa
观测分子量30 kDa
GenBank蛋白编号BC067750
基因名称 ABHD11
Gene ID (NCBI) 83451
RRIDAB_3669471
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Abhydrolase domain-containing protein 11 (ABHD11) is a serine hydrolase enzyme, localized in mitochondria, that can synthesize the endocannabinoid 2-arachidonoyl glycerol (2AG) in vitro.

实验方案

Product Specific Protocols
IF protocol for ABHD11 antibody 25355-1-APDownload protocol
WB protocol for ABHD11 antibody 25355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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