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验证数据展示

  • WB analysis using 86519-4-RR

    WB analysis using 86519-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86519-4-RR (ABCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86519-4-RR

    WB analysis using 86519-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86519-4-RR (ABCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse cerebellum using 86519-4-RR

    IP experiment of mouse cerebellum using 86519-4-RR

    IP result of anti-ABCD2 (IP:86519-4-RR, 4ug; Detection:86519-4-RR 1:2000) with mouse cerebellum tissue lysate 2000 ug.

  • Affinity and Kinetic Characterization of 86519-4-RR

    Affinity and Kinetic Characterization of 86519-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86519-4-RR against Human ABCD2 were performed. The affinity constant is below 1 pM.

ABCD2 Recombinant monoclonal antibody

ABCD2 Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 86519-4-RR

产品说明书

CloneNo. 251255A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

ABCD 2, Adrenoleukodystrophy-like 1, Adrenoleukodystrophy-related protein, ALDRP, ATP-binding cassette sub-family D member 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse adipose tissue, rat cerebellum tissue
Positive IP detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86519-4-RR targets ABCD2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12872

Product name: Recombinant human ABCD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 371-740 aa of BC104901

Sequence: ADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 2
别名 ABCD 2, Adrenoleukodystrophy-like 1, Adrenoleukodystrophy-related protein, ALDRP, ATP-binding cassette sub-family D member 2
计算分子量 740 aa, 83 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC104901
基因名称 ABCD2
Gene ID (NCBI) 225
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD2 is a member of the superfamily of ATP-binding cassette (ABC) transporters, and is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. ABCD2 plays a role in regulation of VLCFAs and energy metabolism, in the degradation and biosynthesis of fatty acids by beta-oxidation (PMID:29397936, 21145416).

实验方案

Product Specific Protocols
IP protocol for ABCD2 antibody 86519-4-RRDownload protocol
WB protocol for ABCD2 antibody 86519-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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