验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, JAR cells, mouse liver tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83038-4-RR targets ABCD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0453 Product name: Recombinant human ABCD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-507 aa of BC025358 Sequence: LMKYVWSASGLLMVAVPIITATGYSESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIERIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIPIVTPSGEVVVASLNIRVEEGMHLLITG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family D (ALD), member 1 |
| 别名 | 230226E12, ABCD 1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1 |
| 计算分子量 | 745 aa, 83 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC025358 |
| 基因名称 | ABCD1 |
| Gene ID (NCBI) | 215 |
| RRID | AB_3670769 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P33897 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for ABCD1 antibody 83038-4-RR | Download protocol |
| IF protocol for ABCD1 antibody 83038-4-RR | Download protocol |
| WB protocol for ABCD1 antibody 83038-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
