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验证数据展示

  • WB analysis of human brain using 11159-1-AP

    WB analysis of human brain using 11159-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 11159-1-AP

    IP experiment of mouse liver using 11159-1-AP

    IP result of anti-ABCD1 (IP:11159-1-AP, 4ug; Detection:11159-1-AP 1:500) with mouse liver tissue lysate 5000ug.

  • IHC staining of human lung cancer using 11159-1-AP

    IHC staining of human lung cancer using 11159-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ABCD1 antibody included
  • IF Staining of HeLa using 11159-1-AP

    IF Staining of HeLa using 11159-1-AP

    Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11159-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCD1 Ready-To-Use IHC Kit. ABCD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11159-1-AP targets ABCD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag1635

Product name: Recombinant human ABCD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 334-507 aa of BC025358

Sequence: LMKYVWSASGLLMVAVPIITATGYSESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIERIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIPIVTPSGEVVVASLNIRVEEGMHLLITG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 1
别名 EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1
计算分子量 745 aa, 83 kDa
观测分子量 75 kDa
GenBank蛋白编号BC025358
基因名称 ABCD1
Gene ID (NCBI) 215
RRIDAB_2219775
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
IF protocol for ABCD1 antibody 11159-1-APDownload protocol
IHC protocol for ABCD1 antibody 11159-1-APDownload protocol
IP protocol for ABCD1 antibody 11159-1-APDownload protocol
WB protocol for ABCD1 antibody 11159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cell Biol Toxicol

CD44-targeted therapy using mP6/Rg3 micelles inhibits oral cancer stem cell proliferation and migration.

Authors - Sijia Cai
View Article
humanWB

Cancers (Basel)

Prostate Cancer Proliferation Is Affected by the Subcellular Localization of MCT2 and Accompanied by Significant Peroxisomal Alterations.

Authors - Isabel Valença
View Article
mouseIF

J Biomed Res

Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis

Authors - Yejin Yao
View Article
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