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验证数据展示

  • WB analysis using 30697-1-AP

    WB analysis using 30697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30697-1-AP (ABCC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 30697-1-AP

    IHC staining of mouse liver using 30697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30697-1-AP (ABCC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30697-1-AP

    IHC staining of mouse liver using 30697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30697-1-AP (ABCC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCC10 Polyclonal antibody

ABCC10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30697-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MRP7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30697-1-AP targets ABCC10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag33719

Product name: Recombinant human ABCC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1203-1492 aa of NM_001198934.1

Sequence: SSFTQTEAMLVSVERLEEYTCDLPQEPQGQPLQLGTGWLTQGGVEFQDVVLAYRPGLPNALDGVTFCVQPGEKLGIVGRTGSGKSSLLLVLFRLLEPSSGRVLLDGVDTSQLELAQLRSQLAIIPQEPFLFSGTVRENLDPQGLHKDRALWQALKQCHLSEVITSMGGLDGELGEGGRSLSLGQRQLLCLARALLTDAKILCIDEATASVDQKTDQLLQQTICKRFANKTVLTIAHRLNTILNSDRVLVLQAGRVVELDSPATLRNQPHSLFQQLLQSSQQGVPASLGGP*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family C (CFTR/MRP), member 10
别名 MRP7
计算分子量161kd
观测分子量~190 kDa
GenBank蛋白编号NM_001198934.1
基因名称 ABCC10
Gene ID (NCBI) 89845
RRIDAB_3086392
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T3U5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATP binding cassette sub-family C member 10 (ABCC10), also named MRP7, is a cellular transmembrane protein transporter and belongs to the ATP-binding cassette (ABC) family. MRP7 is a lipophilic anion pump responsible for the transport of glucuronide E(2)17betaG and can confer resistance to chemotherapy drugs (PMID: 16868766). Since it is glycosylated, the apparent molecular weight of ABCC10 could be variable, ranging from 161 kDa to 191 kDa (PMID: 23087055; PMID: 24103790; PMID: 31338999; PMID: 34631561).

实验方案

Product Specific Protocols
IHC protocol for ABCC10 antibody 30697-1-APDownload protocol
WB protocol for ABCC10 antibody 30697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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