验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue |
| Positive IP detected in | HepG2 cells, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25867-1-AP targets ABCB8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15304 Product name: Recombinant human ABCB8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 329-587 aa of BC141814 Sequence: AFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 8 |
| 别名 | ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1 |
| 计算分子量 | 735 aa, 80 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC141814 |
| 基因名称 | ABCB8 |
| Gene ID (NCBI) | 11194 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NUT2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCB8 is a mitochondrial inner membrane protein and a member of subfamily B of the ABC transporter superfamily. It contains two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs) and transports substrates in an ATP-dependent manner. This protein is a core regulator of mitochondrial iron homeostasis and is responsible for transporting iron-sulfur cluster (Fe-S) precursors from the mitochondrial matrix to the cytosol, thereby maintaining cellular iron homeostasis. Gene knockout or loss of function leads to mitochondrial iron accumulation, reactive oxygen species (ROS) burst, respiratory chain dysfunction, and apoptosis. In addition, ABCB8 is closely associated with iron metabolism-related diseases such as cardiomyopathy and Friedreich's ataxia, and is an important molecule in studies of mitochondrial iron transport, oxidative stress, and tumor metabolism.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ABCB8 antibody 25867-1-AP | Download protocol |
| IP protocol for ABCB8 antibody 25867-1-AP | Download protocol |
| WB protocol for ABCB8 antibody 25867-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
