验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, 37°C incubated K-562 cells, K-562 cellls |
| Positive IP detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
14628-1-AP targets ABCB10 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6203 Product name: Recombinant human ABCB10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 452-738 aa of BC064930 Sequence: YSELMKGLGAGGRLWELLEREPKLPFNEGVILNEKSFQGALEFKNVHFAYPARPEVPIFQDFSLSIPSGSVTALVGPSGSGKSTVLSLLLRLYDPASGTISLDGHDIRQLNPVWLRSKIGTVSQEPILFSCSIAENIAYGADDPSSVTAEEIQRVAEVANAVAFIRNFPQGFNTVVGEKGVLLSGGQKQRIAIARALLKNPKILLLDEATSALDAENEYLVQEALDRLMDGRTVLVIAHRLSTIKNANMVAVLDQGKITEYGKHEELLSKPNGIYRKLMNKQSFISA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 10 |
| 别名 | ABC transporter 10 protein, ABC-me protein, ABC-mitochondrial erythroid protein, ATP-binding cassette sub-family B member 10, mitochondrial, ATP-binding cassette transporter 10 |
| 计算分子量 | 79 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC064930 |
| 基因名称 | ABCB10 |
| Gene ID (NCBI) | 23456 |
| RRID | AB_2220668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRK6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ABCB10 antibody 14628-1-AP | Download protocol |
| WB protocol for ABCB10 antibody 14628-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun ABCB10 depletion reduces unfolded protein response in mitochondria.
| ||
Exp Hematol Abcb10 regulates murine hematopoietic stem cell potential and erythroid differentiation | ||
Mol Cell Unraveling ETC complex I function in ferroptosis reveals a potential ferroptosis-inducing therapeutic strategy for LKB1-deficient cancers |
