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验证数据展示

  • IHC staining of human thymus using 67128-1-Ig

    IHC staining of human thymus using 67128-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67128-1-Ig

    IF Staining of human tonsillitis using 67128-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67128-1-Ig

    IF Staining of human tonsillitis using 67128-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67128-1-Ig

    WB analysis using 67128-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ABCA7 Monoclonal antibody

ABCA7 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67128-1-Ig

产品说明书

CloneNo. 2E1A10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

Autoantigen SS-N, Autoantigen SS N, ATP-binding cassette sub-family A member 7, ABCX, ABCA-SSN

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected in37°C incubated rat brain tissue, Jurkat cells, mouse brain tissue, pig brain tissue, pig thymus tissue, unboiled mouse brain tissue, unboiled rat brain tissue
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67128-1-Ig targets ABCA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ABCA7 fusion protein Ag27499 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family A (ABC1), member 7
别名 Autoantigen SS-N, Autoantigen SS N, ATP-binding cassette sub-family A member 7, ABCX, ABCA-SSN
计算分子量 234 kDa
观测分子量 250 kDa
GenBank蛋白编号NM_019112
基因名称 ABCA7
Gene ID (NCBI) 10347
RRIDAB_2882427
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IZY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCA7 antibody 67128-1-IgDownload protocol
IHC protocol for ABCA7 antibody 67128-1-IgDownload protocol
IF protocol for ABCA7 antibody 67128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols