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ABCA7 Monoclonal antibody

ABCA7 Monoclonal Antibody for IF-P, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2E1A10

Cat No : 67128-1-Ig

Print datasheet

Synonyms

ABCA SSN, ABCA7, ABCX, Autoantigen SS N, FLJ40025, Macrophage ABC transporter

验证数据展示

  • WB analysis of rat brain using 67128-1-Ig

    WB analysis of rat brain using 67128-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thymus using 67128-1-Ig

    IHC staining of human thymus using 67128-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67128-1-Ig

    IF Staining of human tonsillitis using 67128-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67128-1-Ig

    IF Staining of human tonsillitis using 67128-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat brain tissue, Jurkat cells, pig thymus tissue, pig brain tissue, mouse brain tissue
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67128-1-Ig targets ABCA7 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF-P, IHC, WB,ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ABCA7 fusion protein Ag27499 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family A (ABC1), member 7
Synonyms ABCA SSN, ABCA7, ABCX, Autoantigen SS N, FLJ40025, Macrophage ABC transporter
Calculated Molecular Weight 234 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberNM_019112
Gene Symbol ABCA7
Gene ID (NCBI) 10347
RRIDAB_2882427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IZY2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCA7 antibody 67128-1-IgDownload protocol
IHC protocol for ABCA7 antibody 67128-1-IgDownload protocol
IF protocol for ABCA7 antibody 67128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols