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验证数据展示

  • WB analysis using 27450-1-AP (same clone as 27450-1-PBS)

    WB analysis using 27450-1-AP (same clone as 27450-1-PBS)

    unboiled rat lung tissue were subjected to SDS PAGE followed by western blot with 27450-1-AP (ABCA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27450-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 27450-1-AP (same clone as 27450-1-PBS)

    IHC staining of mouse cerebellum using 27450-1-AP (same clone as 27450-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27450-1-AP (ABCA3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27450-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ABCA3 antibody included
  • IHC staining of mouse brain using 27450-1-AP (same clone as 27450-1-PBS)

    IHC staining of mouse brain using 27450-1-AP (same clone as 27450-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27450-1-AP (ABCA3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27450-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ABCA3 antibody included
  • IHC staining of mouse brain using 27450-1-AP (same clone as 27450-1-PBS)

    IHC staining of mouse brain using 27450-1-AP (same clone as 27450-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27450-1-AP (ABCA3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27450-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ABCA3 antibody included

ABCA3 Polyclonal antibody, PBS Only

ABCA3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27450-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

150 Kda mature form, ABC C transporter, ABC3, ABCA 3, ABC-C transporter

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ABCA3 Ready-To-Use IHC Kit. ABCA3 primary antibody included.

产品信息

27450-1-PBS targets ABCA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag24749

Product name: Recombinant human ABCA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1606-1704 aa of BC140895

Sequence: GSGYSLRAKVQSEGQQEALEEFKAFVDLTFPGSVLEDEHQGMVHYHLPGRDLSWAKVFGILEKAKEKYGVDDYSVSQISLEQVFLSFAHLQPPTAEEGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family A (ABC1), member 3
别名 150 Kda mature form, ABC C transporter, ABC3, ABCA 3, ABC-C transporter
观测分子量 140-160 kDa
GenBank蛋白编号BC140895
基因名称 ABCA3
Gene ID (NCBI) 21
RRIDAB_3669603
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99758
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCA3 belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCA3, which is expressed in alveolar type II pneumocytes and localizes predominantly to the limiting membrane of lamellar bodies, is critical for synthesis of surfactant(PMID: 17267394). Mutation of the ABCA3 gene causes fatal surfactant deficiency in newborns(PMID: 15044640).

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