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ABCA1 Polyclonal antibody

ABCA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26564-1-AP

Print datasheet

Synonyms

ABC 1, ABC1, ABCA1, ATP binding cassette 1, CERP, HDLDT1, TGD

验证数据展示

  • WB analysis using 26564-1-AP

    WB analysis using 26564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26564-1-AP (ABCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 26564-1-AP

    IHC staining of mouse lung using 26564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26564-1-AP (ABCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26564-1-AP targets ABCA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen ABCA1 fusion protein Ag24118 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family A (ABC1), member 1
Synonyms ABC 1, ABC1, ABCA1, ATP binding cassette 1, CERP, HDLDT1, TGD
Calculated Molecular Weight 2261 aa, 254 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC141816
Gene Symbol ABCA1
Gene ID (NCBI) 19
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95477
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABCA, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway.

实验方案

Product Specific Protocols
WB protocol for ABCA1 antibody 26564-1-APDownload protocol
IHC protocol for ABCA1 antibody 26564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols