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验证数据展示

  • WB analysis using 10282-1-AP (same clone as 10282-1-PBS)

    WB analysis using 10282-1-AP (same clone as 10282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 10282-1-AP (same clone as 10282-1-PBS)

    WB analysis of HepG2 using 10282-1-AP (same clone as 10282-1-PBS)

    WB result of AATF antibody (10282-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 10282-1-AP (same clone as 10282-1-PBS)

    IHC staining of human ovary cancer using 10282-1-AP (same clone as 10282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10282-1-AP (AATF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 10282-1-AP (same clone as 10282-1-PBS)

    IF Staining of A431 using 10282-1-AP (same clone as 10282-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using AATF antibody (10282-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AATF Polyclonal antibody, PBS Only

AATF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10282-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Apoptosis-antagonizing transcription factor, CHE 1, CHE1, DED, HSPC277

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10282-1-PBS targets AATF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0195

Product name: Recombinant human AATF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-212 aa of BC000591

Sequence: LALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptosis antagonizing transcription factor
别名 Apoptosis-antagonizing transcription factor, CHE 1, CHE1, DED, HSPC277
计算分子量 63 kDa
观测分子量80-95 kDa
GenBank蛋白编号BC000591
基因名称 AATF
Gene ID (NCBI) 26574
RRIDAB_2219887
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY61
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

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