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验证数据展示

  • WB analysis using 14900-1-AP

    WB analysis using 14900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 14900-1-AP

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14900-1-AP

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AARSD1 Polyclonal antibody

AARSD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14900-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AARSD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14900-1-AP targets AARSD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6692

Product name: Recombinant human AARSD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 176-525 aa of BC004172

Sequence: TINDISVLRVTRRGEQADHFTQTPLDPGSQVLVRVDWERRFDHMQQHSGQHLITAVADHLFKLKTTSWELGRFRSAIELDTPSMTAEQVAAIEQSVNEKIRDRLPVNVRELSLDDPEVEQVSGRGLPDDHAGPIRVVNIEGVDSNMCCGTHVSNLSDLQVIKILGTEKGKKNRTNLIFLSGNRVLKWMERSHGTEKALTALLKCGAEDHVEAVKKLQNSTKILQKNNLNLLRDLAVHIAHSLRNSPDWGGVVILHRKEGDSEFMNIIANEIGSEETLLFLTVGDEKGGGLFLLAGPPASVETLGPRVAEVLEGKGAGKKGRFQGKATKMSRRMEAQALLQDYISTQSAKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alanyl-tRNA synthetase domain containing 1
别名 AARSD1
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC004172
基因名称 AARSD1
Gene ID (NCBI) 80755
RRIDAB_2257630
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTE6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARSD1(Alanyl-tRNA synthetase domain-containing protein 1) is also named as alanyl-tRNA editing protein Aarsd1 and belongs to the class-II aminoacyl-tRNA synthetase family. It plays a role in prevention of synthesis of proteins containing errors (mistranslation)(PMID:18172502). It has 3 isoforms produced by alternative splicing with the molecular weight of 45 kDa, 59 kDa and 66 kDa.

实验方案

Product Specific Protocols
WB protocol for AARSD1 antibody 14900-1-APDownload protocol
IHC protocol for AARSD1 antibody 14900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols