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验证数据展示

  • WB analysis using 17394-1-AP

    WB analysis using 17394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17394-1-AP

    WB analysis using 17394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 17394-1-AP

    IHC staining of mouse liver using 17394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17394-1-AP (AlaRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17394-1-AP

    IHC staining of mouse brain using 17394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17394-1-AP (AlaRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 17394-1-AP

    IHC staining of human liver using 17394-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of mouse brain using 17394-1-AP

    IF Staining of mouse brain using 17394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using AlaRS antibody (17394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 17394-1-AP

    IF Staining of mouse liver using 17394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using AlaRS antibody (17394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 17394-1-AP

    WB analysis of HepG2 using 17394-1-AP

    WB result of AlaRS antibody (17394-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HepG2 cells.

  • IP experiment of HepG2 using 17394-1-AP

    IP experiment of HepG2 using 17394-1-AP

    IP result of anti-AlaRS (IP:17394-1-AP, 4ug; Detection:17394-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 17394-1-AP

    IF Staining of HepG2 using 17394-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (17394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

AlaRS Polyclonal antibody

AlaRS Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 17394-1-AP

产品说明书

引用文献(6)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

AARS, AARS1, Alanine--tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase, EC:6.1.1.7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, L02 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17394-1-AP targets AlaRS in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag11294

Product name: Recombinant human AARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC011451

Sequence: MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alanyl-tRNA synthetase
别名 AARS, AARS1, Alanine--tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase, EC:6.1.1.7
计算分子量 968 aa, 107 kDa
观测分子量 107 kDa
GenBank蛋白编号BC011451
基因名称 AlaRS
Gene ID (NCBI) 16
RRIDAB_2219748
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49588
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

实验方案

Product Specific Protocols
IF protocol for AlaRS antibody 17394-1-APDownload protocol
IHC protocol for AlaRS antibody 17394-1-APDownload protocol
IP protocol for AlaRS antibody 17394-1-APDownload protocol
WB protocol for AlaRS antibody 17394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes

Authors - Lingling Li
View Article
humanCoIP

Cancer Lett

K90 lactylation orchestrates YAP nuclear sequestration by impairing binding with exportin CRM1 and enhances HCC malignancy

Authors - Li Wang
View Article
humanWB

Sci Adv

Infection-induced lysine lactylation enables herpesvirus immune evasion

Authors - Matthew D Tyl
  • KD Validated
View Article
humanWB

Cell Discov

NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance

Authors - Jinlong Lin
View Article
humanWB,CoIP

Cell Rep

High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy

Authors - Zhuo Xing
  • KD Validated
View Article
mouseWB

Acta Histochem

AARS1-mediated Osterix lactylation promotes its transcriptional activity during osteoblast differentiation

Authors - Feige Nian
  • KD Validated
View Article
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