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验证数据展示

  • WB analysis of RAW 264.7 using 12392-1-AP

    WB analysis of RAW 264.7 using 12392-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 12392-1-AP

    IHC staining of human cervical cancer using 12392-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 12392-1-AP

    IHC staining of human cervical cancer using 12392-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12392-1-AP

    IF Staining of HeLa using 12392-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

A4GALT Polyclonal antibody

A4GALT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.4.1.228, Alpha4Gal-T1, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, A4GALT1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, K-562 cells, Raji cells
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12392-1-AP targets A4GALT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3069

Product name: Recombinant human A4GALT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-353 aa of BC017068

Sequence: HVVGEPKEKGQLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVESAARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMKMYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha 1,4-galactosyltransferase
别名 EC:2.4.1.228, Alpha4Gal-T1, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, A4GALT1
计算分子量 353 aa, 41 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC017068
基因名称 A4GALT
Gene ID (NCBI) 53947
RRIDAB_2877853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPC4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for A4GALT antibody 12392-1-APDownload protocol
IHC protocol for A4GALT antibody 12392-1-APDownload protocol
WB protocol for A4GALT antibody 12392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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