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Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for WB,IP,IHC,IF/ICC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,IP,IHC,IF/ICC,ELISA

Conjugate

Unconjugated

CloneNo.

4B11F7

Cat No : 66126-1-Ig

Print datasheet

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

验证数据展示

  • WB analysis of HepG2 using 66126-1-Ig

    WB analysis of HepG2 using 66126-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 66126-1-Ig

    IP experiment of human plasma using 66126-1-Ig

    IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug.

  • IF Staining of HepG2 using 66126-1-Ig

    IF Staining of HepG2 using 66126-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver using 66126-1-Ig

    IHC staining of human liver using 66126-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with A2M antibody included
  • IHC staining of human liver using 66126-1-Ig

    IHC staining of human liver using 66126-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with A2M antibody included

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:32000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66126-1-Ig targets Alpha-2-Macroglobulin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,IHC,IF/ICC,ELISA
Tested Reactivity human
Immunogen Alpha-2-Macroglobulin fusion protein Ag19245 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alpha-2-macroglobulin
Synonyms A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M
Calculated Molecular Weight 1474 aa, 163 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC040071
Gene Symbol A2M
Gene ID (NCBI) 2
RRIDAB_2881525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

实验方案

Product Specific Protocols
WB protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
IHC protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
IF protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
IP protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols