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验证数据展示

  • WB analysis using 22647-1-AP (same clone as 22647-1-PBS)

    WB analysis using 22647-1-AP (same clone as 22647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22647-1-AP (A2BP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IP experiment of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IP result of anti-A2BP1 (IP:22647-1-AP, 4ug; Detection:22647-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IHC staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBFOX1/A2BP1 antibody included
  • IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    IF Staining of mouse brain using 22647-1-AP (same clone as 22647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22647-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

A2BP1 Polyclonal antibody, PBS Only

A2BP1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 22647-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBFOX1/A2BP1 Ready-To-Use IHC Kit. RBFOX1/A2BP1 primary antibody included.

产品信息

22647-1-PBS targets A2BP1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18527

Product name: Recombinant human A2BP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC113691

Sequence: MLASQGVLLHPYGVPMIVPAAPYLPGLIQGNQEAAAAPDTMAQPYASAQFAPPQNGIPAEYTAPHPHPAPEYTGQTTVPEHTLNLYPPAQTHSEQSPADTSAQTVSGTATQTDDAAPTDGQPQTQPSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ataxin 2-binding protein 1
别名 FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A
计算分子量 395 aa, 42 kDa
观测分子量 45-70 kDa
GenBank蛋白编号BC113691
基因名称 A2BP1
Gene ID (NCBI) 54715
RRIDAB_2879142
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWB1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.A2BP1 has two mouse isoforms (A2BP1-A016 and -A030) exogenously expressed in COS7 cells showed a molecular mass of 65 kDa, although the calculated molecular masses of A016 and A030 are 40 and 42 kDa, respectively in the course of cell biological study.

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