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验证数据展示

  • WB analysis using 84322-4-RR

    WB analysis using 84322-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84322-4-RR (A1CF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 84322-4-RR

    IHC staining of mouse colon using 84322-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 84322-4-RR

    IHC staining of mouse colon using 84322-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84322-4-RR

    Affinity and Kinetic Characterization of 84322-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84322-4-RR against Human A1CF were performed. The affinity constant is 0.191 nM.

A1CF Recombinant antibody

A1CF Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84322-4-RR

产品说明书

CloneNo. 241644F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64, ACF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84322-4-RR targets A1CF in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 A1CF fusion protein Ag22929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 APOBEC1 complementation factor
别名 APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64, ACF
计算分子量 594 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC054873
基因名称 A1CF
Gene ID (NCBI) 29974
RRIDAB_3671864
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NQ94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for A1CF antibody 84322-4-RRDownload protocol
IHC protocol for A1CF antibody 84322-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols